Objective:To investigate the role of urokinase-type plasminogen activator/urokinase-type plasminogen receptor(u-PA/u-PAR)system in glioma angiogenesis under hypoxic conditions,we studied the effect of glioma-conditioned medium on the hypoxia induced changes in human endothelial-like ECV304 cells proliferation, apoptosis,cord formation in vitro and u-PA/u-PAR expression. Methods:MTT assay was used to examine the changes in cell proliferation.Cell apoptosis was analyzed by Hoechst 33258 staining.Matrigel cord-like formation assay was used to evaluate the angiogenesis ability of ECV304 cells in vitro.Expressions of u-PA/u-PAR mRNA were detected by quantitative real-time RT-PCR. Results:Hypoxia inhibited ECV304 cells proliferation and induced cell apoptosis.Hypoxic conditioned medium(H-CM)while not normoxic conditioned medium(N-CM)of U251 glioma cells partially blocked the effect of hypoxia on ECV304 cells proliferation and apoptosis.H-CM of U251 glioma cells also promoted the cord formation of ECV304 cells seeded on matrigel.When u-PA or u-PAR monoclonal antibodies were added into ECV304 cells culturing medium,cord formation ability was partially inhibited.H-CM of U251 glioma cells induced uPA and uPAR expression in ECV304 cells. Conclusion:These suggest that u-PA/u-PAR system is involved in glioma angiogenesis trigged by hypoxic microenviroment. Objective: To investigate the role of urokinase-type plasminogen activator / urokinase-type plasminogen receptor (u-PA / u-PAR) system in glioma angiogenesis under hypoxic conditions, we studied the effect of glioma-conditioned medium on the hypoxia induced changes in Human endothelial-like ECV304 cells proliferation, apoptosis, cord formation in vitro and u-PA / u-PAR expression. Methods: MTT assay was used to examine the changes in cell proliferation. Cell apoptosis was analyzed by Hoechst 33258 staining. Matrigel cord- like formation assay was used to evaluate the angiogenesis ability of ECV304 cells in vitro. Expressions of u-PA / u-PAR mRNA were detected by quantitative real-time RT-PCR. Results: Hypoxia inhibited ECV304 cells proliferation and induced cell apoptosis. Hypoxic conditioned medium (H-CM) while not normoxic conditioned medium (N-CM) of U251 glioma cells partially blocked the effect of hypoxia on ECV304 cells proliferation and apoptosis. H-CM of U251 glioma cells also promoted the cord formati on of ECV304 cells seeded on matrigel. Wu u-PA or u-PAR monoclonal antibodies were added into ECV304 cell culturing medium, cord formation ability was partially inhibited. H-CM of U251 glioma cells induced uPA and uPAR expression in ECV304 cells. : These suggest that u-PA / u-PAR system is involved in glioma angiogenesis trigged by hypoxic microenviroment.