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目的比较还原型谷胱甘肽(GSH)对KLE和HEC-1-b子宫内膜癌细胞生长的影响,并探讨其机制。方法①用GSH处理KLE和HEC-1-b子宫内膜癌细胞,噻唑蓝(MTT)法检测两型细胞的增殖水平;②用5 mmol/L GSH处理KLE和HEC-1-b子宫内膜癌细胞,流式细胞仪检测细胞内活性氧(ROS)水平,酶标仪检测细胞内GSH和氧化型谷胱甘肽(GSSG)水平,分光光度计检测细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性。结果在不进行处理的情况下,KLE子宫内膜癌细胞较HEC-1-b子宫内膜癌细胞的ROS水平及CAT活性较低,GSH水平、GSH/GSSG比值及SOD和GSH-Px活性较高(P<0.01)。用GSH处理后,KLE子宫内膜癌细胞生长加快,细胞内ROS和GSH水平及GSH/GSSG比值下降,SOD、CAT和GSH-Px活性降低(P<0.01);HEC-1-b子宫内膜癌细胞生长被抑制,细胞内ROS、GSH及GSSG水平下降,GSH/GSSG比值明显升高,CAT和GSH-Px活性降低(P<0.01)。结论GSH对两种子宫内膜癌细胞生长有明显不同的影响,与两种细胞内ROS水平和抗氧化系统活性有密切的关系。
Objective To compare the effects of reduced glutathione (GSH) on the growth of endometrial carcinoma cells in KLE and HEC-1-b cells and to explore its mechanism. Methods ① The proliferation of KLE and HEC-1-b endometrial carcinoma cells was detected by GSH and the proliferation of two types of cells was detected by MTT assay. ② The endometrial cells of KLE and HEC-1-b endometrium were treated with 5 mmol / L GSH The levels of intracellular reactive oxygen species (ROS) were detected by flow cytometry, the levels of intracellular GSH and glutathione (GSSG) were detected by microplate reader. The levels of superoxide dismutase (SOD) , Catalase (CAT) and glutathione peroxidase (GSH-Px) activity. Results Compared with HEC-1-b endometrial carcinoma cells, the levels of ROS and CAT activity in KLE endometrial cancer cells were lower than those in HEC-1-b endometrial cancer cells. GSH level, GSH / GSSG ratio and SOD and GSH-Px activities High (P <0.01). After treatment with GSH, the growth of KLE endometrial cancer cells accelerated, ROS and GSH levels and GSH / GSSG ratio decreased, and the activities of SOD, CAT and GSH-Px decreased (P <0.01); HEC-1-b endometrium The growth of cancer cells was inhibited. The levels of ROS, GSH and GSSG were decreased, the ratio of GSH / GSSG was significantly increased and the activities of CAT and GSH-Px were decreased (P <0.01). Conclusions GSH can significantly affect the growth of two endometrial cancer cells, which is closely related to the level of ROS and the activity of antioxidant system.