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目的:探讨溶血磷脂酸受体3(LPAR3)、同源框基因-11(HOXA-11)在IVF-ET患者的子宫内膜组织着床窗口期的表达及意义。方法:采用免疫组织化学SP和半定量反转录-聚合酶链反应(RT-PCR)两种方法,检测LPAR3和HOXA-11在拟行IVF-ET患者排卵后5~7天(胚胎着床窗期)的子宫内膜组织中的表达。根据助孕后的结局,将153例因输卵管因素不孕的患者分为妊娠组67例和未妊娠组86例。结果:LPAR3在妊娠组腺上皮的表达显著高于未妊娠组,差异有统计学意义(P<0.01);HOXA-11在妊娠组腺上皮的表达均显著低于未妊娠组,在间质细胞中的表达均显著高于未妊娠组,差异有统计学意义(P<0.01)。结论:LPAR3在着床窗期子宫内膜腺上皮的表达减少,与HOXA-11在着床窗期子宫内膜腺上皮表达增加和间质细胞HOXA-11表达减少可能是导致子宫内膜容受性下降的原因之一。LPAR3、HOXA-11可以作为预测子宫内膜容受性的参考指标。
Objective: To investigate the expression and significance of lysophosphatidic acid receptor 3 (LPAR3) and homeobox gene 11 (HOXA-11) in the implantation window of endometrial tissue in patients with IVF-ET. Methods: The levels of LPAR3 and HOXA-11 in ovariectomized IVF-ET patients were determined 5 to 7 days after ovulation by using immunohistochemical SP and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) Window) endometrial tissue expression. According to the post-pregnancy outcome, 153 patients with tubal infertility were divided into pregnancy group (67 cases) and non-pregnancy group (86 cases). Results: The expression of LPAR3 in glandular epithelium in pregnancy group was significantly higher than that in non-pregnant group (P <0.01). The expression of HOXA-11 in glandular epithelium in pregnancy group was significantly lower than that in non-pregnant group The expression was significantly higher in non-pregnant group, the difference was statistically significant (P <0.01). CONCLUSION: The decreased expression of LPAR3 in the endometrial glandular epithelium during the implantation window and the increased expression of HOXA-11 in the endometrial glandular epithelium during the implantation window and the reduction in the expression of HOXA-11 in the stromal cells may be responsible for the endometrial receptivity One of the reasons for the decline in sex. LPAR3, HOXA-11 can be used as a reference to predict endometrial receptivity.