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目的·· :探讨吗啡依赖对小鼠海马不同亚区一氧化氮合酶(NOS)活性的影响。方法·· :以剂量递增法皮下注射吗啡建立吗啡依赖小鼠模型 ,采用还原型辅酶Ⅱ -黄递酶(NADPH -d)组织化学法显示吗啡依赖组、纳洛酮催促戒断组和正常对照组小鼠海马CA1区、CA3区和齿状回NOS阳性神经元。结果·· :与对照组相比 ,吗啡依赖组和纳洛酮催促戒断组海马CA1区和齿状回NOS阳性神经元的数目均明显减少 (P<0.01) ,纳洛酮催促戒断组更为明显 ,而在CA3区无明显改变。结论·· :吗啡依赖和纳洛酮催促戒断小鼠海马CA1区和齿状回NOS活性降低 ,提示NO合成的能力下降 ,戒断期下降更明显。这些变化可能是吗啡依赖造成学习记忆功能下降的原因之一。
Objective: To investigate the effects of morphine dependence on nitric oxide synthase (NOS) activity in different sub-regions of hippocampus of mice. Methods · · · · · · · · · · · · · · · · · · · · Morphine-dependent mouse model was established by subcutaneous injection of morphine by dose escalation. Morphine-dependent group and naloxone-induced withdrawal group were compared with those of normal control by NADPH-d histochemical method Groups of hippocampal CA1 area, CA3 area and dentate gyrus NOS positive neurons. Results · Compared with the control group, the number of NOS positive neurons in hippocampal CA1 area and dentate gyrus in morphine dependent group and naloxone group were significantly decreased (P <0.01), and naloxone induced withdrawal group More obvious, but no obvious change in CA3 area. Conclusions · · ·: Morphine dependence and naloxone induced withdrawal hippocampal CA1 area and dentate gyrus NOS activity decreased, suggesting that the ability of NO synthesis decreased, the withdrawal period is more obvious. These changes may be one of the reasons for the decline of learning and memory function caused by morphine dependence.