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根据已知鸟氨酸转氨酶(δ-OAT)的保守序列设计简并引物,并利用RT-PCR和RACE技术从‘迎霜’茶树中克隆获得鸟氨酸转氨酶基因,命名为Csδ-OAT,其Gen Bank登录号为KJ641844。该基因c DNA全长为1 865 bp,编码473个氨基酸,理论等电点为7.19,推测分子量为52.3 k D。序列比对分析结果表明,Csδ-OAT主要功能域保守性较高,存在典型的PLP结合位点;系统进化树分析显示,Csδ-OAT的进化符合传统的生物学分类,与其他双子叶植物具有同一起源。q RT-PCR分析结果表明,Csδ-OAT基因的表达存在明显的组织特异性,在花中表达最高,其次是叶片,而在其他组织器官中表达较低。此外,Csδ-OAT基因受高盐、低温、干旱、ABA和氧化胁迫处理的诱导,表明其参与了茶树体内各种非生物胁迫响应的过程。
Degenerate primers were designed based on the conserved sequences of known ornithine aminotransferases (δ-OAT), and the ornithine aminotransferase gene was cloned from ’Yingcao’ tea plant by RT-PCR and RACE technology and named as Csδ-OAT Gen Bank accession number is KJ641844. The full-length cDNA of this gene was 1 865 bp, encoding 473 amino acids with a theoretical isoelectric point of 7.19 and a predicted molecular weight of 52.3 kD. Sequence alignment analysis showed that the main functional domains of Csδ-OAT were highly conserved, with typical PLP binding sites. Phylogenetic tree analysis showed that the evolution of Csδ-OAT was in accordance with the traditional taxonomic classification, Same origin q RT-PCR analysis showed that Csδ-OAT gene expression was significantly tissue-specific, highest expression in flowers, followed by leaves, and lower expression in other tissues and organs. In addition, Csδ-OAT gene was induced by high salt, low temperature, drought, ABA and oxidative stress, which indicated that Csδ-OAT was involved in various abiotic stress responses in tea plants.