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目的:探讨硫胺素对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的急性帕金森病(PD)小鼠模型脑室管膜下区(SVZ)神经发生的保护作用。方法:给予C57BL小鼠腹腔注射MPTP,1 d内连续4次,每次注射间隔2 h,每次20 mg.kg-1,制作PD模型(PD组);在PD组基础上给予小鼠腹腔注射硫胺素100 mg.kg-1.d-1(从MPTP使用前1周开始注射至MPTP使用后1周)设为硫胺素干预PD组(PDT组)。另设正常对照组(Ctrl组)和正常硫胺素组(CtrlT组)。利用免疫荧光染色法检测各组小鼠脑SVZ区5-溴脱氧尿嘧啶核苷(BrdU)和双皮质蛋白(Dcx)阳性细胞的分布和数目。结果:PD组SVZ区BrdU和Dcx阳性细胞与Ctrl组比较均显著减少(P<0.05);PDT组SVZ区BrdU和Dcx阳性细胞与PD组比较明显增加(P<0.05)。结论:MPTP诱导的急性PD小鼠模型脑SVZ区神经发生明显减少,硫胺素对PD小鼠模型脑SVZ区受损的神经发生具有保护作用。
AIM: To investigate the effect of thiamine on the subventricular zone (SVZ) of acute Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Neuroprotective effects. Methods: The C57BL mice were injected intraperitoneally with MPTP for 4 times a day for 2 h intervals, each time with a dose of 20 mg.kg-1 to make PD model (PD group). On the basis of PD group, mice were given intraperitoneal Injection of thiamine 100 mg.kg-1.d-1 (1 week after MPTP injection 1 week prior to MPTP administration) was set as thiamine intervention PD group (PDT group). Another normal control group (Ctrl group) and normal thiamine group (CtrlT group). Immunofluorescence staining was used to detect the distribution and number of 5-bromodeoxyuridine (BrdU) and cortex-like protein (Dcx) positive cells in SVZ of the mice in each group. Results: BrdU and Dcx positive cells in SVZ of PD group were significantly decreased compared with Ctrl group (P <0.05). BrdU and Dcx positive cells in PDZ group were significantly increased compared with PD group (P <0.05). CONCLUSIONS: MPTP-induced acute PD mice have significantly reduced neurogenesis in the SVZ area and thiamine has a protective effect on impaired neurogenesis in the SVZ area of PD mice.