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目的:探讨阿托伐他汀对内皮细胞微粒(EMPs)诱导的人脐静脉内皮细胞(HUVECs)表达血管细胞粘附分子(VCAM)-1和细胞间粘附分子(ICAM)-1的影响。方法:取生长良好的第4,5代人脐静脉内皮细胞,将细胞分为3大组:对照组、EMPs组、EMPs+阿托伐他汀组。对照组加入培养基,EMPs组以不同浓度的EMPs(0/mL,1×102/mL,1×103/mL,1×104/mL,1×105/mL)与HUVECs共同孵育24 h,EMPs+阿托伐他汀组以不同浓度的阿托伐他汀(0.05,0.1,1.0,10μmol.L-1)与HUVECs作用1 h后,加入105/mL EMPs共同孵育24 h。分别采用实时荧光定量聚合酶链反应和蛋白免疫印迹方法检测VCAM-1和ICAM-1 mRNA和蛋白的表达。结果:HUVECs受EMPs刺激后,VCAM-1和ICAM-1 mRNA及蛋白表达呈浓度依赖性增加,阿托伐他汀可不同程度上抑制EMPs的作用。结论:阿托伐他汀抗动脉粥样硬化作用可能部分与抑制EMPs诱导的内皮细胞VCAM-1和ICAM-1的表达有关。
Objective: To investigate the effect of atorvastatin on the expressions of vascular cell adhesion molecule (VCAM) -1 and intercellular adhesion molecule (ICAM) -1 in human umbilical vein endothelial cells (HUVECs) induced by endothelial cell particles (EMPs). Methods: The well-grown human umbilical vein endothelial cells of the fourth and fifth generations were divided into three groups: control group, EMPs group and EMPs + atorvastatin group. The control group was added with medium, EMPs group were incubated with HUVECs for 24 h with different concentrations of EMPs (0 / mL, 1 × 102 / mL, 1 × 103 / mL, 1 × 104 / mL, 1 × 105 / mL) Atorvastatin group and different concentrations of atorvastatin (0.05, 0.1, 1.0, 10μmol.L-1) and HUVECs for 1 h, after adding 105 / mL EMPs incubated for 24 h. The expression of VCAM-1 and ICAM-1 mRNA and protein were detected by real-time fluorescence quantitative polymerase chain reaction and Western blot respectively. Results: The mRNA and protein expressions of VCAM-1 and ICAM-1 in HUVECs stimulated by EMPs increased in a concentration-dependent manner. Atorvastatin inhibited the effects of EMPs to varying degrees. CONCLUSIONS: Atorvastatin may contribute to the suppression of atherosclerosis partly by inhibiting the expression of VCAM-1 and ICAM-1 induced by EMPs in endothelial cells.