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世界范围内过敏症状的流行趋势日益严峻,水稻作为单子叶模式植物和人类主要粮食作物,对水稻中致敏性物质的研究具有重要意义。但目前基于水稻全基因组水平预测和分析过敏原的研究尚缺少相关报道。本研究利用生物信息学方法在全基因组层面上,在水稻基因组数据库中和过敏原数据库中,共筛选和获得了657个过敏原基因/蛋白(属于109个蛋白家族),并进一步对这些候选的水稻过敏原基因/蛋白进行了表达模式、家族分类和功能预测分析;以及在进化上分析了水稻过敏原基因/蛋白家族成员的同源蛋白在低等到高等植物中分布情况,结果表明致敏性可能随着基因家族中成员的功能分化而变化;进一步结合水稻花粉和种子转录组芯片数据,细化分析和获得了148个水稻花粉过敏原和116个水稻种子过敏原,并在基因家族和功能分类上进行了对比分析。本研究结果初步明确了水稻过敏原基因/蛋白家族的进化特点、组织表达特性和生物学功能,为进一步研究水稻以及其他单子叶植物中的过敏原奠定了基础。
The epidemic trend of allergy symptoms in the world is becoming more and more serious. Rice, as monocotyledonous plant and major human food crops, is of great significance to the research of allergenic substances in rice. However, there are still few reports on the prediction and analysis of allergens based on genome-wide rice level. In this study, we used bioinformatics methods to screen and obtain 657 allergen genes / proteins (belonging to 109 protein families) at genome-wide level, in rice genome database and allergen database, and further analyze these candidate Rice allergen gene / protein expression patterns, family classification and functional prediction analysis; and evolutionary analysis of rice allergen allergen / protein family members homologous protein distribution in lower to higher plants, the results show that allergenicity Which may change with the functional differentiation of members of the gene family. Based on the chip data of rice pollen and seed transcriptome, 148 rice pollen allergens and 116 rice seed allergens were analyzed and obtained in detail. In the gene family and function Classification conducted a comparative analysis. The results of this study initially identified the evolutionary characteristics, tissue expression characteristics and biological functions of the rice allergen gene / protein family, which laid the foundation for the further study of allergens in rice and other monocotyledons.