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目的:探讨膀胱尿路上皮癌(UCCB)组织中CIP2A的表达及其在膀胱尿路上皮癌发生发展中的意义。方法:应用免疫组化法及逆转录聚合酶链反应法,对48例临床分期为Tis~T1期的膀胱癌组织标本中CIP2A的表达进行检测。病理分级G1级23例,G2级17例,G3级8例;复发性膀胱癌17例;15例非肿瘤膀胱组织作对照应。结果:15例非肿瘤膀胱组织CIP2A表达均为阴性;48例膀胱癌组织中有19例可见CIP2A表达;复发的膀胱癌组织中CIP2A阳性表达率(58.82%,10/17)明显高于初发的膀胱癌组织(29.03%,9/31),两组CIP2A表达阳性率有显著性差异(P<0.05);CIP2A表达率在不同分级的肿瘤组织中无明显差异(P>0.05)。两年随访发现:CIP2A表达阳性的肿瘤病人复发率高(13/19,68.42%),与CIP2A表达阴性者的复发率(11/29,37.93%)相比有显著性差异(P<0.05)。结论:CIP2A与膀胱癌复发有关,检测膀胱癌组织中CIP2A表达有望作为膀胱癌诊断的一种指标,在膀胱肿瘤的预后判断中起重要作用。
Objective: To investigate the expression of CIP2A in bladder urothelial carcinoma (UCCB) and its significance in the development of bladder urothelial carcinoma. Methods: The expression of CIP2A in 48 specimens of bladder cancer with clinical stage of Tis ~ T1 was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT - PCR). Pathological grade G1 grade 23 cases, G2 grade 17 cases, G3 grade 8 cases; recurrent bladder cancer 17 cases; 15 cases of non-tumor bladder tissue as a control. Results: The expression of CIP2A in all 15 non-tumorous bladder tissues was negative. CIP2A expression was found in 19 of 48 bladder cancer tissues (58.82%, 10/17) (29.03%, 9/31). There was significant difference between the two groups (P <0.05). The expression rate of CIP2A had no significant difference among different grading tumor tissues (P> 0.05). Two-year follow-up found that patients with positive CIP2A expression had a higher recurrence rate (13/19, 68.42%) than those with negative CIP2A expression (11/29, 37.93%) (P 0.05) . Conclusion: CIP2A is associated with the recurrence of bladder cancer. Detecting the expression of CIP2A in bladder cancer is expected to be an indicator of bladder cancer diagnosis and plays an important role in the prognosis of bladder cancer.