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以笃斯越橘优系为试材,研究不同培养基、激素、pH值、光照时间、温度对其试管苗生根的影响。试验结果表明,笃斯越橘试管苗最优生根培养基为改良1/2WPM+0.10 mg/L IBA+1.5mg/L IAA+15 g/L蔗糖+8 g/L琼脂,pH值5.4、光照时间8小时/天、培养温度为25℃时,生根率最高,生根率在90%以上。
The effects of different media, hormones, pH value, light time and temperature on the rooting of test-tube seedling were studied with the test of Vaccinium. The results showed that the optimum medium for rooting of testis was 1 / 2WPM + 0.10 mg / L IBA + 1.5 mg / L IAA + 15 g / L sucrose + 8 g / L agar at pH 5.4, Time 8 hours / day, the culture temperature is 25 ℃, the highest rooting rate, rooting rate above 90%.