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近年来我院采用盐析法浓缩胃液后作IgG和IgA测定,发现胃癌患者胃液中的含量高于胃部良性疾患的含量,现将结果初步报告如下。 材料和方法 一、材料:(一)饱和硫酸铵(用15N NH_4OH调正pH7.0备用);(二)IgG、IgA抗血清及正常人参考血清(均为上海医化所出品);(三)胃液标本为我院内科、外科手术前空腹插胃管采集和胃镜室纤维内窥镜检查时用尼龙管抽取胃液送检。 二、方法:胃液有酸者用碱液调正其pH近中性,放低温冰箱过夜,翌日置室温中融化并加压过滤
In recent years, our hospital used the method of concentration of gastric juice after salting out to measure IgG and IgA. It was found that the content of gastric juice in gastric cancer patients was higher than that in benign stomach diseases. The results are now reported as follows. Materials and Methods 1. Materials: (a) Saturated ammonium sulfate (adjusted to pH 7.0 with 15N NH 4OH); (b) IgG, IgA antiserum, and reference serum for normal people (all produced by Shanghai Medical and Chemical Institute); Gastric fluid specimens were collected from the Department of Internal Medicine in our hospital, collected before the surgical intervention of fasting stomach tubes, and examined with endoscopic fiberoptic endoscopy. Second, the method: Gastric acid with lye to adjust its pH near neutral, put the refrigerator at low temperature overnight, day and night at room temperature and pressure filtration