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以 9311为轮回亲本 ,以IRBB2 1为抗白叶枯病供体亲本 ,通过杂交和三次回交以及两次自交 ,结合农艺性状的选择 ,建立起具 4 4个株系的BC3F3群体。分别用连锁标记 pTA2 4 8和基因内STS标记MXA2 1对抗性基因Xa2 1进行前景选择。用均匀分布在水稻全基因组的 2 4 8个SSR标记 ,进行两亲本之间的多态性分析 ,获得 76个具多态性的SSR标记 ,用于背景选择。结果选到基因型背景回复到轮回亲本的 73 6 8% - 88 16 % ,且Xa2 1基因纯合的株系 4个 ,分别为M 0 71、M 0 82、M0 86和M 0 87。以Xa2 1的鉴别菌系菲律宾 6号小种PXO99接种鉴定表明 ,9311为感 (S) ,IRBB2 1为抗 (R) ,中选株系M 0 71和M 0 82的抗性与IRBB2 1相同 ,为抗 (R)级 ,M 0 86和M 0 87抗性略低于IRBB2 1,为抗到中抗(R/MR) ,这两个株系内单株之间抗性有差异。农艺性状考察结果显示 ,中选株系与轮回亲本 9311相似。以中选株系M 0 86与培矮 6 4S配制的F1和原组合两优培九 (培矮 6 4S/ 9311)进行比较 ,对白叶枯病的抗性有了明显提高 ,综合农艺性状相似。论文就白叶枯病抗性基因的综合利用以及育种方法进行了讨论。
With 9311 as the recurrent parent and IRBB2 1 as the donor of the bacterial blight, BC4F3 population with 4 4 lines was established by crossing and three backcrosses and two inbreds with the selection of agronomic traits. The foreground selection was carried out with the linkage marker pTA2 4 8 and the intragenic STS marker MXA2 1 resistance gene Xa2 1, respectively. Using the 288 SSR markers evenly distributed in the genome of rice, polymorphism analysis between the two parents was conducted, and 76 polymorphic SSR markers were obtained for background selection. Results The genotypes were 73.68% -88.16% of the recurrent parents and 4 were homozygous for the Xa21 gene, with M 0 71, M 0 82, M 0 86 and M 0 87 respectively. The identification of Xa2 1 -associated Philippines PXO99 inoculation showed that 9311 was susceptible (S) and IRBB2 1 was anti-(R). The selected lines M 0 71 and M 0 82 had the same resistance as IRBB2 1, To be resistant (R), the resistance of M 0 86 and M 0 87 was slightly lower than that of IRBB2 1 and was resistant to medium resistance (R / MR). The results of agronomic traits showed that the selected plants were similar to the recurrent parent 9311. Compared with the F1 and the original Liangyoupeijiu (Pei’ai 6 4S / 9311) formulated with the selected lines M 0 86 and Pei’ai 6 4S, the resistance to bacterial leaf blight was significantly improved and the synthetic agronomic traits were similar. The paper discussed the comprehensive utilization of bacterial blight resistance genes and breeding methods.