目的探讨慢性肾功能衰竭 (CRF)患者及血液透析 (HD)过程中多肽生长因子及凋亡因子的含量变化及相互关系。　方法采用流式细胞术及放射免疫分析法分别测定 CRF及 HD前后外周血单核细胞 (PBMC)中 Fas抗原 (CD95 )、线粒体膜蛋白 (Apo2 .7)、抗凋亡因子 (BC1- 2 )蛋白的阳性细胞数及血清中转化生长因子α(TGF-α)、类胰岛素样生长因子 (IGF- )的含量。结果 CRF (非透析组 ) CD95、Apo2 .7、IGF- 明显高于对照组。BC1- 2与 TGF-α含量均明显低于正常人 (P <0 .0 1)。HD后 CD95和 Apo2 .7表达明显高于 HD前 (P<0 .0 5 ) ,BC1- 2蛋白在 HD前后无差异 (P>0 .0 5 )。结论多肽生长因子与凋亡因子在肾脏细胞凋亡过程中起重要作用 Objective To investigate the changes of the content of polypeptide growth factor and apoptosis factor in patients with chronic renal failure (CRF) and hemodialysis (HD) and their relationship. Methods The expressions of Fas antigen (CD95), mitochondrial membrane protein (Apo2.7) and anti-apoptotic factor (BC1-2) in peripheral blood mononuclear cells (PBMCs) before and after CRF and HD were measured by flow cytometry and radioimmunoassay. The number of positive cells and the content of TGF-α and IGF-1 in serum. Results CRF (non-dialysis group) CD95, Apo2 .7, IGF- was significantly higher than the control group. BC1-2 and TGF-α levels were significantly lower than normal (P <0.01). The expression of CD95 and Apo2.7 in HD was significantly higher than that in HD before HD (P <0.05), but there was no difference in BC1-2 before and after HD (P> 0.05). Conclusion Polypeptide growth factor and apoptosis factor play an important role in the process of renal cell apoptosis