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目的鉴定单纯疱疹病毒HSV糖蛋白CgC一个短肽模拟表位mgC基因作为HSVDNA表位疫苗的可能性。方法将mgC基因克隆入真核载体pcDNA3.1中,并在CHO细胞中表达,免疫荧光法鉴定表达效果。DNA免疫不同种属小鼠,ELISA法观察HSVgC特异结合抗体的诱生。结果含mgC的真核载体在哺乳动物细胞中可以成功表达外源蛋白。重组载体DNA在BALB/c小鼠体内可诱导抗天然HSVgC蛋白的抗体,而在C57BL鼠体内未见明显的抗体反应。结论成功构建了可表达模拟表位mgC的真核表达载体。证明了应用gC模拟短肽基因作为DNA免疫的基因源是可行的,但其对不同种属动物的反应性不同,提示DNA免疫还应针对不同免疫对象调整免疫策略。这一研究为HSV多表位组合多肽疫苗的研制提供了一个新的备选组份。
OBJECTIVE: To identify the possibility that a short peptide mimotope mgC gene of herpes simplex virus HSV glycoprotein CgC could serve as an HSVDNA epitope vaccine. Methods The mgC gene was cloned into eukaryotic vector pcDNA3.1 and expressed in CHO cells. The expression of mgC gene was identified by immunofluorescence. DNA was used to immunize mice of different species, and the induction of HSVgC specific binding antibody was observed by ELISA. Results The eukaryotic vector containing mgC successfully expressed the foreign protein in mammalian cells. Recombinant vector DNA induced anti-native HSVgC antibody in BALB / c mice, whereas no significant antibody response was found in C57BL mice. Conclusion The eukaryotic expression vector expressing mimic epitope mgC was successfully constructed. It is proved that it is feasible to use gC to simulate the short peptide gene as the gene source of DNA immunization, but it has different reactivity to different species of animals, suggesting that DNA immunization should also adjust immunization strategies for different immune targets. This study provides a new candidate for the development of HSV polytope-binding peptide vaccine.