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目的探讨姜黄素对糖尿病肾病大鼠的肾内转化生长因子表达的影响。方法采用一次性腹腔注射链脲佐菌素58 mg·kg-1(5.8 m L·kg-1)建立糖尿病肾病大鼠模型。实验组灌胃姜黄素混悬液200 mg·kg-1,正常组与模型组则灌服等体积的1%羟甲基纤维素钠。用免疫组化法观察肾小球、肾小管区域转化生长因子的表达。结果与模型组比较,实验组大鼠24 h尿微量白蛋白、空腹血糖、血浆转化生长因子的含量均明显降低(P<0.01),肾小球、肾小管区域转化生长因子的表达也有显著降低(P<0.01)。结论姜黄素能下调转化生长因子在血清及肾的表达,对糖尿病肾病大鼠的肾有一定的保护作用。
Objective To investigate the effect of curcumin on the expression of renal transforming growth factor (TGF) in rats with diabetic nephropathy. Methods A rat model of diabetic nephropathy was established by intraperitoneal injection of streptozotocin at a dose of 58 mg · kg-1 (5.8 m L · kg-1). The experimental group was given intragastric administration of curcumin suspension 200 mg · kg-1, while the normal group and model group were fed the same volume of 1% sodium carboxymethylcellulose. Immunohistochemistry was used to observe the expression of transforming growth factor (TGF) in glomeruli and tubules. Results Compared with model group, 24 h urine microalbumin, fasting blood glucose and plasma transforming growth factor were significantly decreased in experimental group (P <0.01), while glomerular and tubular regions also had significantly lower expression of transforming growth factor (P <0.01). Conclusion Curcumin can down-regulate the expression of TGF-β in serum and kidneys and protect the kidney of diabetic nephropathy rats.