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作者用氨基酸分析仪测定了中国台北的健康人、极度近视患者和老年性白内障患者血清和房水中的氨基酸和有关化合物的含量。方法:标准氨基酸从Pierce化学公司购入。临测定前将谷氨酰胺和天冬酰胺(E.Merck)加入标准氨基酸混合液中。供分析氨基酸的全部试剂,均从E.Merck或Sigma化学公司购入,全部试验过程用去离子蒸馏水。样品收集:健康人血清样品由医学院外观健康的一年级生和研究生捐赠,年龄18～23岁。
The authors used amino acid analyzer to determine the contents of amino acids and related compounds in serum and aqueous humor of healthy people, extreme myopia patients and senile cataract in Chinese Taipei. Method: Standard amino acids were purchased from Pierce Chemical Company. Glutamine and asparagine (E. Merck) were added to the standard amino acid mixture prior to assay. All reagents for the analysis of amino acids were purchased from E. Merck or Sigma Chemical Company, using deionized distilled water for all of the experiments. Sample collection: Healthy human serum samples were donated by first-graders and post-graduate students with a healthy appearance in the medical school, aged 18 to 23 years.