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车前草样品在16～18℃通风干燥后,将根、根茎、叶柄、花序柄、花穗(含花、果实、萼片和花柄)分别粉碎成粉末状,置硅胶干燥器中保存。桃叶珊瑚甙的HPLC测定条件样品溶液的制备:取约100mg的样品粉末,精密称定,加入50％甲醇水溶液5.0ml,在室温下用超声波振荡提取30min,提取液离心分离后,上清液通过微孔滤膜(0.2μm),吸取5μl注入到HPLC分析,以外标峰面积法定量。对照品桃叶珊瑚甙(日本米山药品工业)。 HPLC分析条件:岛津LC-3A液相色谱仪,Unisil C_(18)柱(10μm),4.6×250mm,流动相3％乙腈水溶液,流速1.5ml/min,检测210nm,桃叶珊瑚甙的保留时间为10.8min。
After the plantain samples were air-dried at 16-18°C, the roots, rhizomes, petioles, inflorescence stems, flower spikes (flowers, fruits, sepals, and pedicels) were crushed into powder and stored in a silica gel desiccator. Determination of HPLC conditions of Peach Leaf Coral A sample solution preparation: Take about 100mg of sample powder, accurately weighed, add 50% methanol aqueous solution 5.0ml, extract with ultrasonic vibration at room temperature for 30min, the extract is centrifuged, the supernatant Through a microporous membrane (0.2 μm), 5 μl was pipetted into the HPLC analysis and quantified by the peak area method. Controls Peach Leaf Coral (Japan Miyama Pharmaceutical Industry). HPLC analysis conditions: Shimadzu LC-3A liquid chromatograph, Unisil C 18 column (10 μm), 4.6 250 mm, mobile phase 3% acetonitrile aqueous solution, flow rate 1.5 ml/min, detection at 210 nm, retention of Peachleaf coral reefs The time is 10.8 minutes.