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1991年,我们在进行莱姆病自然疫源地调查时。从捕自辽东山区桓仁、宽甸县的蜱、鼠中分离到多株伯氏疏螺旋体,从而证实辽宁省存在莱姆病自然疫源地。一、方法:采用笼捕法捕获野栖鼠类;用布旗法采集野外游离蜱;分离培养用BSK培基。二、结果:从7组38匹全沟硬蜱(Ixodes persulcatus)分离出6株伯氏疏螺旋体,以组计算,分离率为85.7％;从16组342匹嗜群血蜱(Haemaphysalis concinno)分离出4株伯氏疏螺旋体,
In 1991, we conducted a survey of natural foci of Lyme disease. Multiple strains of Borrelia burgdorferi were isolated from ticks and mice captured from Huanren and Kuandian counties in the mountains of Liaodong, so as to confirm the presence of natural Lyme disease in Liaoning Province. First, the method: cage capture method to capture wild rats; cloth flag method to capture wild ticks; isolated and cultured with BSK culture medium. Results: Six strains of Borrelia burgdorferi isolated from 38 populations of Ixodes persulcatus in seven groups were isolated and sequenced. The isolation rate was 85.7%. From 342 Haemaphysalis concinno Four strains of Borrelia burgdorferi,