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目的：观察人牙周膜细胞生物学特性表达。方法：采用细胞克隆技术，以细胞的牙骨质附着蛋白（ Ｃ Ａ Ｐ） 亲和力、碱性磷酸酶（ Ａ Ｌ Ｐ） 表达率和细胞的钙化能力为指标，对５ 名患者的５ 个正常前磨牙的牙周膜（ Ｐ Ｄ Ｌ） 细胞进行了初步的鉴定。结果：在接种的６６９ 个克隆细胞中，仅有４３ ．６ ％ 的克隆细胞具有持续增殖潜力。在这些生长克隆细胞中，非钙化细胞所占比例要多于钙化细胞，但这两类细胞中分别存在着 Ａ Ｌ Ｐ 表达和 Ｃ Ａ Ｐ 亲和力有着不同表现的细胞。结论：牙周膜中存在有不同表现型的细胞。提示牙周组织再生是一复杂的过程。这些细胞在牙周组织再生中起着什么作用，尚待进一步研究。
Objective: To observe the biological characteristics of human periodontal ligament cells. Methods: Cell cloning technique was used to detect the expression of C A P, the expression of ALP and the calcification ability of cells in 5 normal subjects Periodontal ligament (PDL) cells of molars were preliminarily identified. Results: Of the 669 colonies inoculated, only 43 were found. Six percent of cloned cells have a constant proliferative potential. In these growing clonal cells, the proportion of non-calcified cells accounted for more than calcified cells, but there are two types of cells, respectively, A L P expression and C A P affinity have different manifestations of the cells. Conclusion: There are different phenotype cells in periodontal ligament. Suggest that periodontal tissue regeneration is a complex process. What role these cells play in periodontal regeneration remains to be further studied.