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目的:从核桃仁内隔膜中提取并测定总黄酮含量,选择最佳提取工艺条件。方法:以芦丁为对照品,用分光光度法在最大吸收波长510nm对其含量进行测定。结果:测得样品中总黄酮含量C=6.430%,最佳提取工艺:乙醇浓度为75%、料液比为1∶40、超声提取时间为60min、超声提取温度为80℃。结论:选用芦丁为对照品应用于紫外分光光度法测定核桃仁内隔膜总黄酮含量准确度较高,方法简单,可作为测定核桃仁内隔膜中黄酮含量的一种手段。
Objective: To extract and determine the content of total flavonoids from the inner membrane of walnut and select the best extraction conditions. Methods: Rutin was used as reference substance, its content was determined by spectrophotometry at the maximum absorption wavelength of 510nm. Results: The content of total flavonoids in samples was 6.430%. The optimum extraction conditions were: ethanol concentration 75%, solid-liquid ratio 1:40, ultrasonic extraction time 60 min and ultrasonic extraction temperature 80 ℃. CONCLUSION: The method of using rutin as reference substance in UV spectrophotometry for determination of total flavonoids content in walnut kernels has high accuracy. The method is simple and can be used as a means to determine flavonoid content in walnut inner membrane.