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目的通过对中国人非小细胞肺癌(NSCLC)患者肿瘤组织中环氧化酶-2(COX-2)、血管内皮细胞生长因子(VEGF)、热休克蛋白27(HSP27)和P53表达的检测,观察这4种蛋白质在NSCLC肿瘤组织中的表达情况,分析其与性别、年龄、病理类型、肿瘤细胞分化程度、肿瘤直径、淋巴结转移的相关性,为临床判断和有效控制NSCLC提供有价值的参考。方法采用免疫组织化学法检测50例NSCLC患者肿瘤组织中COX-2、VEGF、HSP27和P53的表达,采用原位杂交法检测COX-2mRNA的表达。结果COX-2、VEGF、HSP27、P53单一指标阳性均与性别、年龄、组织学分类、肿瘤直径、N分期、肿瘤细胞分化程度无关。COX-2与淋巴结转移有关(偏相关系数为0.3237,P=0.028),而VEGF、HSP27、P53与淋巴结转移无关。COX-2和P53或COX-2和VEGF共同高表达在N2期的NSCLC中明显多于N0和N1期;COX-2、P53和VEGF共同高表达或其中两种蛋白质高表达在N2期的NSCLC中明显多于N0和N1期(P=0.002)。HSP27高表达虽然与淋巴结转移无关,但与COX-2高表达有关(偏相关系数为0.5143)。结论COX-2、P53和VEGF共同高表达或其中两种蛋白质高表达与NSCLC淋巴结转移密切相关。COX-2与NSCLC淋巴结转移有关。
Objective To investigate the expression of COX-2, VEGF, HSP27 and P53 in Chinese patients with non-small cell lung cancer (NSCLC) 4 protein expression in NSCLC tumor tissue, and analyze its correlation with gender, age, pathological type, tumor cell differentiation, tumor diameter, lymph node metastasis, provide valuable reference for clinical judgment and effective control of NSCLC. Methods The expressions of COX-2, VEGF, HSP27 and P53 in 50 NSCLC patients were detected by immunohistochemistry. The expression of COX-2 mRNA was detected by in situ hybridization. Results The positive expression of COX-2, VEGF, HSP27 and P53 was not related to gender, age, histological classification, tumor diameter, N stage and tumor cell differentiation. COX-2 was associated with lymph node metastasis (partial correlation coefficient was 0.3237, P = 0.028), while VEGF, HSP27, P53 had no correlation with lymph node metastasis. Co-expression of COX-2 and P53 or COX-2 and VEGF were significantly higher in N2 stage NSCLC than in N0 and N1 stage. COX-2, P53 and VEGF were overexpressed or both of them were highly expressed in stage N2 NSCLC In the N0 and N1 phases (P = 0.002). Although HSP27 overexpression was not related to lymph node metastasis, it was correlated with the high expression of COX-2 (partial correlation coefficient was 0.5143). Conclusion The high expression of COX-2, P53 and VEGF, or the high expression of two of them, are closely related to lymph node metastasis in NSCLC. COX-2 is associated with lymph node metastasis in NSCLC.