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以温室嫁接黄瓜为试材,研究高温和加富CO2条件下叶片净光合速率、Rubisco活性、Rubisco活化酶活性、蒸腾速率、胞间CO2浓度的日变化规律及其相互关系。旨为高温季节温室黄瓜高产优质栽培提供理论依据和实践支持。研究结果表明:1高温+CO2条件下,净光合速率、Rubisco活性和Rubisco活化酶活性存在明显的光合日变化,均呈双峰曲线,存在“午休”现象,但与对照相比全天的数值明显增加,其中净光合速率在10:00、11:00、12:00、13:00、14:00和15:00时分别比对照高22%、47%、37%、27%、39%和28%;Rubisco活性在9:00、10:00、11:00、12:00、13:00、14:00和15:00时分别比对照高51%、69%、70%、42%、77%、61%和59%;RCA活性在8:00、9:00、10:00、11:00、12:00、13:00、14:00、15:00和16:00时比对照分别升高了40%、39%、23%、41%、43%、31%、60%、60%和54%;2 Rubisco活化酶对光合速率的直接影响较小,其本质是调节Rubisco活性,从而影响光合作用;3高温+CO2可以有效提高Rubisco活化酶活力以及胞间CO2浓度,从而提高光合速率。
The greenhouse cucumber was used to study the diurnal variation of net photosynthetic rate, Rubisco activity, Rubisco activase activity, transpiration rate and intercellular CO2 concentration under high temperature and CO2 enrichment and their correlations. To provide theoretical basis and practical support for high-yielding and high-quality cucumber cultivation in hothouse season. The results showed that there were obvious diurnal photosynthetic changes of net photosynthetic rate, Rubisco activity and Rubisco activase activity under the condition of high temperature and CO2, both showed a double peaked curve with the phenomenon of “lunch break”, but compared with the control The net photosynthetic rate was 22%, 47%, 37%, 27% higher than the control at 10: 00,11: 00,12: 00,13: 00,14: 00 and 15:00 respectively, 39% and 28% respectively. Rubisco activity was 51%, 69%, 70% higher than that of the control at 9:00, 10:00, 11:00, 12:00, 13:00, 42%, 77%, 61% and 59%; RCA activity was at 8:00, 9:00, 10:00, 11:00, 12:00, 13:00, 14:00, 15:00 and 16:00 Rubisco activase had a lower direct effect on photosynthetic rate by 40%, 39%, 23%, 41%, 43%, 31%, 60%, 60% and 54% Regulation of Rubisco activity, thus affecting photosynthesis; 3 high temperature + CO2 can effectively improve Rubisco activase activity and intercellular CO2 concentration, thereby increasing photosynthetic rate.