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目的:建立用高效液相色谱同时测定复方川芎胶囊中阿魏酸和川芎嗪含量的方法。方法:用Inertsil ODS2 C_(18)柱(150 mm×4.6 mm,5μm),流动相:甲醇-0.5%冰醋酸溶液(25:75),检测波长:280 nm,流速:1.0 ml·min~(-1)。结果:阿魏酸和川芎嗪的线性范围分别为4.4～44μg·ml~(-1)(r=0.999 9)和2.0～20.2μg·ml~(-1)(r=0.999 8),平均回收率分别为100.6% (RSD 2.1%)和100.9%(RSD 2.2%)(n=6)。结论:该法简单、快速、重复性好,可用于样品的含量测定及质量控制。
Objective: To establish a method for simultaneous determination of ferulic acid and tetramethylpyrazine in compound Chuanxiong capsules by high performance liquid chromatography. METHODS: Using Inertsil ODS2 C18 column (150 mm×4.6 mm, 5 μm), mobile phase: methanol-0.5% acetic acid solution (25:75), detection wavelength: 280 nm, flow rate: 1.0 ml·min~( -1). Results: The linear ranges of ferulic acid and tetramethylpyrazine were 4.4-44 μg·ml -1 (r=0.999 9) and 2.0～20.2 μg·ml -1 (r=0.999 8), respectively. The rates were 100.6% (RSD 2.1%) and 100.9% (RSD 2.2%) (n=6), respectively. Conclusion: This method is simple, rapid, and reproducible. It can be used for the determination of samples and quality control.