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在研究天然杀伤细胞(NK)和淋巴因子激活杀伤细胞(LAK)活性实验中,由于其活性受时间等因素的影响,所测结果波动较大,为了避免此种实验误差,首先将不同条件获得的淋巴细胞冻存,然后同时复苏,在相同条件下测结果。冻存和复苏可以降低淋巴细胞的细胞毒活性,但有研究显示,复苏的淋巴细胞若在37℃预先孵育18小时(称为静止作用)能够恢复NK 活性。观察了自IL-2治疗之前、之间和之后癌症病人获得的淋巴细胞,以及体外IL-2激活淋巴细胞的合适静止作用实验条件。
In the study of natural killer (NK) and lymphokine-activated killer (LAK) activity experiments, because of its activity by the time and other factors, the measured results fluctuate greatly, in order to avoid such experimental error, first of all different conditions were obtained Lymphocytes were cryopreserved, then resuscitated at the same time, and the results were measured under the same conditions. Cryopreservation and resuscitation can reduce the cytotoxic activity of lymphocytes, but studies have shown that resuscitated lymphocytes can restore NK activity if preincubated for 18 hours at 37 ° C (called quiescent action). Lymphocytes obtained from cancer patients before, during and after IL-2 treatment and appropriate resting conditions for IL-2-activated lymphocytes in vitro were observed.