论文部分内容阅读
目的研究硫化氢(H2S)对人肝癌HepG2细胞迁移及MMP-9表达的影响。方法划痕愈合和Transwell实验检测H2S供体硫氢化钠(NaHS)对HepG2细胞迁移的影响;Western blot检测NaHS对HepG2细胞MMP-9表达的影响。结果划痕实验结果显示,200和400μmol·L-1的NaHS作用HepG2细胞16 h后,较未处理组划痕距离明显增加(P<0.05),表明H2S可抑制HepG2细胞的愈合能力。Transwell实验结果显示,100、200和400μmol·L-1的NaHS作用HepG2细胞24 h后,穿膜细胞数分别为(18.3±1.16)、(16.3±1.52)、(12.3±0.58)个,较未处理组的(24.0±1.73)个明显减少(P<0.05),表明H2S可抑制HepG2细胞迁移。Western blot结果显示,100、200和400μmol·L-1的NaHS作用HepG2细胞24 h后,MMP-9蛋白表达明显降低(P<0.05)。结论 H2S可抑制人肝癌HepG2细胞的迁移,其机制可能与下调MMP-9有关。
Objective To study the effect of hydrogen sulfide (H2S) on the migration of human hepatocellular carcinoma HepG2 cells and the expression of MMP-9. Methods Scratch healing and Transwell assay were used to detect the effect of NaHS on the migration of HepG2 cells. Western blot was used to detect the effect of NaHS on the expression of MMP-9 in HepG2 cells. Results Scratch assay showed that the HepG2 cells treated with 200 and 400 μmol·L-1 of NaHS significantly increased the scratch distance compared with the untreated group (P <0.05) after 16 h treatment, indicating that H2S inhibited HepG2 cell healing. The results of Transwell assay showed that the numbers of transmembrane cells in HepG2 cells treated with 100, 200 and 400 μmol·L-1 NaHS for 24 h were (18.3 ± 1.16), (16.3 ± 1.52) and (12.3 ± 0.58), respectively (24.0 ± 1.73) in treatment group decreased significantly (P <0.05), indicating that H2S can inhibit HepG2 cell migration. Western blot results showed that the expression of MMP-9 protein in HepG2 cells treated with NaHS at 100, 200 and 400 μmol·L -1 for 24 h decreased significantly (P <0.05). Conclusion H2S can inhibit the migration of HepG2 cells, which may be related to the down-regulation of MMP-9.