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目的:观察金锁二仙饮对恐伤肾小鼠的影响并探讨其补肾填精作用机理。方法:将60只小鼠随机分为正常组、模型组、金匮肾气丸组以及金锁二仙饮高、中和低剂量组,共6组。除正常组外,其余5组制造“恐伤肾”肾精亏虚动物模型,连续21天,造模第8天开始模型组每天灌胃生理盐水(0.2mL/10g)、金匮肾气丸组灌胃金匮肾气丸混悬液(2.7g·kg-1·d-1)、金锁二仙饮高、中和低剂量组分别以金锁二仙饮(36、18、9g·kg-1·d-1)灌胃,共14天。观察动物一般情况,体重、性腺重量及其指数,血清睾酮(T)、精子生成素(FSH)和间质细胞刺激素(LH)含量的变化。结果:金锁二仙饮能够改善小鼠肾精亏虚症状,增加小鼠体重及性腺重量,提高血清T、FSH、LH水平。结论:金锁二仙饮可能通过对下丘脑-垂体-性腺轴激素水平的合成和释放的调节,发挥补肾填精作用。
Objective: To observe the effects of Jinsuo Erxian drink on the kidney of mice with acute kidney injury and to explore its mechanism of action of kidney tonifying and filling. Methods: Sixty mice were randomly divided into six groups: normal group, model group, Jingui Shenqi Pill group and Jinshou Erxian high, medium and low dose groups. In addition to the normal group, the remaining 5 groups were made animal models of “kidney injury” kidney deficiency, for 21 consecutive days, the model group began daily gavage with saline (0.2mL / 10g) on the 8th day, The Qiongzhu Decoction suspension (2.7g · kg-1 · d-1) was administered orally to the Qiwan pill group, the Jinzhong Erxian Drink (36,18, 9g · kg-1 · d-1) intragastrically for 14 days. The changes of general condition, body weight, gonad weight and index, serum testosterone (T), spermatogenic hormone (FSH) and interstitial cell stimulating hormone (LH) were observed. Results: Jinsuo Erxian drink can improve kidney deficiency syndrome in mice, increase body weight and gonad weight, and increase serum T, FSH and LH levels. Conclusion: Jinsuo Erxian Decoction can play the role of tonifying the kidney through the regulation of hypothalamus-pituitary-gonadal axis hormone synthesis and release.