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以‘桂味’荔枝为试材,花后45 d用双层牛皮纸对整个果穗进行完全遮光处理,14 d后(采收前7 d)解除遮光,比较遮光和解除遮光处理果皮着色以及花色素苷生物合成途径中LcUFGT等8个结构基因和调控基因LcMYB表达的变化,探索光照调控荔枝果实花色素苷合成和着色的分子机制。研究发现:遮光处理显著抑制了果皮花色素苷的合成,延缓了果实的正常着色;解除遮光后,花色素苷迅速合成,7 d后呈现荔枝典型的红色;遮光处理抑制了花色素苷生物合成途径中LcUFGT等8个重要结构基因和调控基因LcMYB1的正常表达;解除遮光后LcUFGT和LcMYB1等基因的表达量均有不同程度的增加,其中LcDFR、LcF3’H、LcUFGT和LcMYB1的表达与花色素苷含量的变化规律基本一致。可见,荔枝果皮花色素苷积累和着色依赖光照,花色素苷生物合成途径中主要的结构基因和转录因子都可以被光诱导,其中LcDFR、LcF3’H、LcUFGT和LcMYB1可能在光照调控荔枝果实色泽形成中扮演更为重要的角色。
The cinnamon litchi was used as test material, and the whole ear was completely shaded with double-decker kraft paper 45 days after anthesis. After 14 days (7 days before harvest), the shading was lifted, and shading and shading were performed to compare the color of peel and anthocyanidin Glycoside biosynthesis pathway LcUFGT and other eight structural genes and regulatory gene LcMYB expression changes to explore the light regulation of litchi fruit anthocyanin synthesis and coloring of the molecular mechanism. The results showed that shading treatment significantly inhibited the synthesis of anthocyanin in fruit peel and delayed the normal coloring of fruit. After shading, the anthocyanins were rapidly synthesized, and the typical red of litchi appeared after 7 days. Shading treatment inhibited the biosynthesis of anthocyanin Pathway, and LcMYB1. The expression of LcFGF, LcMYB1 and LcMYB1 were all increased to some extent. The expression of LcDFR, LcF3’H, LcUFGT and LcMYB1 were positively correlated with the expression of LcMYB1, Glycoside content of the law of change is basically the same. It can be seen that the accumulation and coloring of anthocyanin in litchi pericarp relied on light and the major structural genes and transcription factors in the pathway of anthocyanin biosynthesis could be induced by light. Among them, LcDFR, LcF3’H, LcUFGT and LcMYB1 might be involved in the regulation of litchi fruit color Formation plays a more important role.