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目的研究南海海域Euplexaura rhipidalis种属柳珊瑚的丙酮提取物及其不同溶剂萃取部位的抗肿瘤活性,探讨最强活性部位可能的作用机制。方法丙酮提取物及其萃取部位作用于人肺癌细胞A549和人肝癌细胞Hep G2,采用磺酰罗丹明B(SRB)法检测细胞增殖抑制率;甲醇重溶部位(EM)以低、中、高浓度作用于A549和Hep G2细胞,采用流式细胞仪检测细胞凋亡比率和细胞周期分布。结果 EM的体外抗肿瘤活性最强,对A549和Hep G2细胞的IC50值均小于10μg·ml-1,优于或接近阳性对照5-FU;EM能显著诱导细胞凋亡并呈现良好的浓度依赖性,中高浓度时对细胞G2/M或S期有周期阻滞作用。结论甲醇重溶部位是柳珊瑚Euplexaura rhipidalis主要的抗肿瘤活性部位,能抑制A549和Hep G2细胞的增殖,具有诱导凋亡、阻滞周期的作用。
Objective To investigate the antitumor activity of acetone extracts from different species of Euplexium rapididalis in the South China Sea and their solvent extraction sites and to explore the possible mechanism of action of the strongest active site. Methods Acetone extract and its extract were applied to human lung cancer cell line A549 and human hepatocellular carcinoma cell line Hep G2. Cell proliferation inhibition rate was determined by sulforhodamine B (SRB) assay. Methanol re-dissolved fraction (EM) Concentration on A549 and Hep G2 cells, the apoptosis rate and cell cycle distribution were detected by flow cytometry. Results The anti-tumor activity of EM was the strongest in vitro, and the IC50 value of A549 and Hep G2 cells was less than 10μg · ml-1, which was better than or close to the positive control 5-FU. EM could induce apoptosis significantly and showed a good concentration dependence Sexual, medium and high concentrations of G2 / M or S cell cycle arrest effect. Conclusion Methanol re-dissolving site is the main anti-tumor active site of Euplexia rhipidalis, which inhibits the proliferation of A549 and Hep G2 cells and induces apoptosis and arrests the cycle.