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目的:探讨红芪多糖-3(HPS-3)对2型糖尿病(T2DM)大鼠糖脂代谢和胰腺组织病理改变的影响。方法:建立T2DM大鼠模型,按体重随机分为T2DM模型组、HPS-3不同剂量组(50、100、200 mg/kg)及二甲双胍组(200 mg/kg),均灌胃给药,5周后取血清测血糖、糖耐量、血脂(总胆固醇﹑甘油三酯﹑高密度脂蛋白胆固醇﹑低密度脂蛋白胆固醇);取肝大叶,用蒽酮-硫酸法测肝糖原;取胰腺切片染色,观察胰岛β细胞病理改变。结果:与模型组比较,HPS-3各剂量组(50、100、200 mg/kg)均可降低T2DM大鼠空腹血糖和糖耐量实验中口服葡萄糖后2h的血糖,改善了大鼠的糖耐量减退,其中以200 mg/kg最为明显。HPS-3能调节T2DM大鼠的血脂紊乱,抑制总胆固醇、甘油三酯、低密度脂蛋白胆固醇升高和高密度脂蛋白胆固醇降低;同时,在一定程度上有增加T2DM大鼠肝糖原含量的趋势和修复受损胰岛β细胞的作用。结论:HPS-3能降低血糖,对T2DM大鼠的糖脂代谢紊乱有一定的调节作用,能促进T2DM大鼠肝糖原的合成,修复受损的胰岛β细胞,减轻T2DM大鼠胰岛素抵抗。
Objective: To investigate the effect of Hs-3 on the metabolism of glucose and lipid and the pathological changes of pancreas in type 2 diabetes mellitus (T2DM) rats. Methods: T2DM model rats were randomly divided into T2DM model group, HPS-3 different dose groups (50,100,200 mg / kg) and metformin group (200 mg / kg) Blood samples were collected for blood glucose, glucose tolerance, blood lipids (total cholesterol, triglycerides, HDL-C and LDL-C); liver lobules were harvested for hepatic glycogen analysis using anthrone- Slice stained, observed islet β cell pathological changes. Results: Compared with the model group, the HPS-3 dose groups (50, 100, 200 mg / kg) all reduced the blood glucose level at 2 h after oral administration of glucose in T2DM rats and improved the glucose tolerance Decreased, of which 200 mg / kg most obvious. HPS-3 can regulate dyslipidemia in T2DM rats, inhibit the increase of total cholesterol, triglyceride, low density lipoprotein cholesterol and high density lipoprotein cholesterol; at the same time, it can increase the content of hepatic glycogen in T2DM rats The tendency and role of repairing damaged islet beta cells. CONCLUSION: HPS-3 can lower blood glucose and regulate the glucose and lipid metabolism in T2DM rats. It can promote the synthesis of hepatic glycogen in T2DM rats, repair damaged islet β cells and relieve insulin resistance in T2DM rats.