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目的:研究不同剂量Nd:YAG激光照射后,牙本质小管封闭深度及不同深度内钙磷比值的变化,得出Nd:YAG激光治疗牙本质过敏症的最佳参数。方法:酸蚀法建立牙本质敏感模型,应用不同参数的Nd:YAG激光照射样本,A组(0 mj,0 Hz,对照组);B组(30 mj,5 Hz);C组(30 mj,10 Hz);D组(50 mj,5 Hz);E组(50 mj,10 Hz)。照射完成后将样本纵剖,扫描电镜观察照射前后样本牙本质小管封闭深度并用X-线能谱分析不同深度处样本钙磷比值变化。结果:扫描电镜见B组牙本质小管表面封闭,近表面处小管直径比对照组变小。C、D两组牙本质小管熔融达一定厚度,且牙本质结构完整。E组牙本质熔融厚度达25μm,但熔融区中有微孔产生。X线能谱分析各组样本5μm、20μm深处的钙磷比值,差异无统计学意义。结论:Nd:YAG激光牙本质脱敏的较为理想参数为(30 mj,10Hz)和(50 mj,5 Hz)。
OBJECTIVE: To study the changes of the ratio of calcium and phosphorus in the depth of dentinal tubule occlusion and the depth of dentin tubule after Nd: YAG laser irradiation. The optimal parameters of Nd: YAG laser for dentine hypersensitivity were obtained. Methods: The model of dentin hypersensitivity was established by acid etching. Samples were irradiated with different parameters of Nd: YAG laser in group A (0 mj, 0 Hz, control group), group B (30 mj, 5 Hz), group C , 10 Hz); Group D (50 mj, 5 Hz); Group E (50 mj, 10 Hz). After irradiation, the samples were longitudinally dissected and the depth of occlusion of the dentinal tubules before and after irradiation was observed by scanning electron microscopy. The changes of calcium-phosphorus ratio at different depths were analyzed by X-ray energy dispersive spectroscopy. Results: The surface of dentin tubule in group B was closed by SEM, and the diameter of tubule in the proximal surface was smaller than that of the control group. C and D dentin tubules were melted to a certain thickness, and the dentin structure was intact. Group E dentin melt thickness of 25μm, but there are micropores in the melt zone. X-ray energy spectrum analysis of each sample 5μm, 20μm depth of calcium and phosphorus ratio, the difference was not statistically significant. Conclusion: The ideal parameters of Nd: YAG laser dentin desensitization are (30 mj, 10 Hz) and (50 mj, 5 Hz).