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目的观察日本血吸虫感染ICR小鼠后不同时期T淋巴细胞中杀伤性T细胞(Tc)亚型Tc1和Tc2比例的动态变化,并分析两者分别与Th1和Th2细胞比例变化的相关性。方法分别取日本血吸虫感染0、3、5、8、13周的小鼠脾脏淋巴细胞,采用流式细胞术检测各个时期Tc1、Tc2和Th1、Th2细胞亚群分别占T淋巴细胞的比例。结果与0周对照比较,感染3、5、8、13周小鼠T淋巴细胞中Tc1细胞比例显著增高(P均<0.01),感染5、8、13周小鼠T淋巴细胞中Tc2细胞比例显著增高(P均<0.01)。比较Tc1和Tc2细胞比例在相同时间点的增速发现,感染3周时Tc1细胞比例增高最显著,而感染5周时Tc2细胞比例的增高最显著。感染各个时间点CD3+T细胞中的Th1细胞比例与Tc1细胞比例平行增长,且两者呈正相关(r=0.978,P=0.004);CD3+T细胞中的Th2细胞比例与Tc2细胞比例平行增长,且两者呈正相关(r=0.974,P=0.005)。体外可溶性成虫抗原(SWA)刺激脾细胞可优势增加T淋巴细胞中的Tc1细胞比例(P<0.01),而可溶性虫卵抗原(SEA)刺激脾细胞可优势增加T淋巴细胞中的Tc2细胞比例(P<0.01)。结论日本血吸虫感染不同阶段,小鼠T淋巴细胞中的Tc1和Tc2细胞比例均显著升高,并分别与Th1和Th2细胞比例呈正相关。在感染3周时主要以Tc1细胞比例升高为主,而感染5周时主要以Tc2细胞比例升高为主。SWA可优势诱导Tc1细胞,而SEA可优势诱导Tc2细胞。
Objective To observe the dynamic changes of the Tc1 and Tc2 ratios of Tc subtypes in T lymphocytes of Schistosoma japonicum infected with ICR mice at different periods and to analyze the correlation between them and the ratio of Th1 and Th2 cells, respectively. Methods The spleen lymphocytes of mice infected with Schistosoma japonicum at 0, 3, 5, 8 and 13 weeks were collected respectively. The proportion of T lymphocyte subsets Tc1, Tc2, Th1 and Th2 were detected by flow cytometry. Results Compared with the 0 week control group, the Tc1 cells in T lymphocytes in 3, 5, 8, 13 weeks infected mice were significantly increased (all P <0.01). The percentage of Tc2 cells in T lymphocytes in mice infected with 5, Significantly higher (P <0.01). Comparison of Tc1 and Tc2 cell growth rate at the same time point found that the highest proportion of Tc1 cells increased at 3 weeks of infection, and the highest increase of Tc2 cells ratio at 5 weeks of infection. The proportion of Th1 cells in CD3 + T cells at each time point increased in parallel with the proportion of Tc1 cells, and the two showed a positive correlation (r = 0.978, P = 0.004). The proportion of Th2 cells in CD3 + T cells and Tc2 cells increased in parallel , And there was a positive correlation between them (r = 0.974, P = 0.005). Stimulation of spleen cells by SWA in vitro could increase the proportion of Tc1 cells in T lymphocytes (P <0.01), while stimulation of spleen cells by soluble egg antigen (SEA) could significantly increase the proportion of Tc2 cells in T lymphocytes P <0.01). Conclusion In different stages of Schistosoma japonicum infection, the proportion of Tc1 and Tc2 cells in T lymphocytes of mice increased significantly, which was positively correlated with the ratio of Th1 and Th2 cells, respectively. At 3 weeks of infection, the main proportion of Tc1 cells was increased, while the main proportion of Tc2 cells was increased at 5 weeks of infection. Tc1 cells were predominantly induced by SWA, while Tc2 cells were predominantly induced by SEA.