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为了研究敌敌畏(dichlorvos,DDVP)对人肾细胞的毒性效应。应用MTT比色法检测敌敌畏对人肾细胞系293T细胞增殖的影响,流式细胞仪检测293T细胞的凋亡情况,荧光二氯荧光素二乙酸(DCF-DA)测定细胞内的活性氧(reactive oxidative species,ROS)水平。结果表明:用浓度为0.5 mmol/L、1 mmol/L、5 mmol/L、10 mmol/L、15 mmol/L和20 mmol/L的敌敌畏分别处理293T细胞1 h后发现敌敌畏对293T的生长抑制具有剂量效应,计算得到1 h的IC_(50)值为10.406 mmol/L;不同浓度敌敌畏(0.5 mmol/L,5 mmol/L,10 mmol/L)处理1 h 293T细胞发现细胞产生明显的凋亡现象,随着处理浓度的增加,细胞的凋亡率升高,检测细胞内活性氧水平发现细胞内活性氧水平也随之升高。结果提示敌敌畏能够通过凋亡途径抑制293T细胞的增殖,其作用机制可能是通过增加293T细胞内活性氧水平,促进细胞凋亡,进而抑制细胞增殖。
To investigate the toxic effects of dichlorvos (DDVP) on human renal cells. The effects of dichlorvos on the proliferation of human renal cell line 293T cells were detected by MTT colorimetric assay. The apoptosis of 293T cells was detected by flow cytometry. The levels of reactive oxygen species (ROS) in cells were determined by Fluorescein dichlorofluorescein diacetate (DCF-DA) oxidative species, ROS) levels. The results showed that the growth of 293T cells was induced by the treatment of 293T cells with dichlorvos at concentrations of 0.5 mmol / L, 1 mmol / L, 5 mmol / L, 10 mmol / L, 15 mmol / L and 20 mmol / (IC50) value of 10.406 mmol / L at 1 h was calculated. The cells treated with different concentrations of dichlorvos (0.5 mmol / L, 5 mmol / L, 10 mmol / L) Apoptosis, with the increase of treatment concentration, cell apoptosis rate, detection of intracellular reactive oxygen species level found within the cell reactive oxygen species also increased. The results suggest that dichlorvos can inhibit the proliferation of 293T cells through the apoptotic pathway. The mechanism may be through increasing the level of reactive oxygen species (ROS) in 293T cells and promoting cell apoptosis, thereby inhibiting cell proliferation.