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目的研究突触结构形态特征，探讨自发性高血压大鼠（ＳＨＲ）中缝背核的突触前、突触后调节机制。方法电镜观察ＳＨＲ和正常对照大鼠（ＷＫＹ）中缝背核的超微结构。按Ｂｏｎｄｏｋ的方法，进行突触结构参数的形态计量学测量和数据分析。结果在ＳＨＲ中缝背核中突触接触区的数密度和面密度均大于ＷＫＹ（Ｐ＜０．０１），并且ＳＨＲ突触接触区面密度的增加是由于ＳＨＲ突触数目增多的结果。ＳＨＲ中缝背核突触后致密物偶见突触下致密小体。中缝背核存在典型穿孔突触，ＳＨＲ穿孔突触可见多个孔洞和突触小泡偏心现象。结论ＳＨＲ中缝背核有较多的突触参与局部整合环路。ＳＨＲ突触下致密小体可能与兴奋性氨基酸合成增加、谷氨酸受体激活以及兴奋性冲动增加有关。ＳＨＲ穿孔突触可能与突触活性、重建速度以及接触面积增加有关
Objective To study the morphological characteristics of synaptic structure and to explore the mechanism of presynaptic and postsynaptic modulation in dorsal raphe nucleus in spontaneously hypertensive rats (SHR). Methods The ultrastructure of dorsal raphe nucleus in SHR and normal control rats (WKY) were observed by electron microscope. According to Bondok’s method, morphometric measurements and data analysis of synaptic structural parameters were performed. Results The number density and surface density of synaptic contact in SHR were higher than those in WKY (P <0.01), and the increase in the density of SHR synaptic contact was due to the increase of the number of SHR synapses. Synaptic cleft synaptic density in SHR occasionally seen synaptic dense bodies. There are typical perforation synapses in the dorsal raphe nuclei. Multiple holes and synaptic vesicle eccentricity can be observed in SHR perforation synapses. Conclusion There are more synapses in the dorsal raphe nucleus of SHR involved in the local integration loop. SHR synaptic compact may be associated with increased excitatory amino acid synthesis, glutamate receptor activation and increased excitatory impulses. SHR perforation synapses may be related to synaptic activity, speed of reconstruction, and increased contact area