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为优化筛选青贮玉米秸秆与牛粪混合消化时的总固体浓度 (TS), 在37℃中温条件下, 系统研究TS分别为10％、12％和14％时的产沼气性能、发酵液性质以及秸秆消化前后的理化特征.结果表明:青贮玉米秸秆与牛粪在TS为12％时的混合发酵产气效果优于10％和14％时, 该条件下秸秆中纤维素和半纤维素的降解率分别为17.05％和45.13％.秸秆发酵前后的微观形貌和纤维素结晶度分析发现, 秸秆的木质纤维结构变化明显, 纤维素结晶度减小, 且TS为12％时秸秆消化降解最严重, 结晶指数最小.总之, 适宜青贮玉米秸秆与牛粪混合发酵的TS值为12％, 且消化过程中半纤维素最易于分解, 但木质素几乎不被降解.“,”This study aimed to investigate fermentation parameters through anaerobic co-digestion of maize straw silage (MSS) and cattle manure with different initial total solids concentrations (TS) of 10％, 12％ and 14％. The experiment was carried out in 1.5 L batch digesters at mesophilic temperature (37 ℃) to evaluate the effects of different TS on anaerobic cogroups when the TS was 12％. Under this condition, the degradation efficiencies of cellulose and hemicelluloses were17.05％ and 45.13％ respectively. To analyze the micromorphology change of before and after straw fermentation and the crystallinity of cellulose is found that wood fiber structure of straw has very big change but the crystallinity of cellulose is reduced. Also, the digestion and degradation of straw with the TS of 12％ was most complete and had lowest crystallization index. In conclusion, the anaerobic digestion process was favored when the TS was 12％ in which hemicellulose is the easiest to decompose but lignin is degraded hardly.