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目的:研究低剂量无细胞短棒状杆菌纳米级制剂(NCPP)对刀豆蛋白 A(Con A)诱导的小鼠急性肝损伤的保护作用及机理。方法:BALB/c 小鼠腹腔注射 NCPP(0.01 mg·只)后第7 d 以 Con A(25μg·g~(-1))尾静脉注射诱导小鼠急性肝损伤模型。测定血清转氨酶(ALT/AST)及肝脏内的病理变化。同时设置 PBS 预处理对照组和 Con A 模型对照组,每组8只小鼠。用流式细胞仪检测肝脏内自然杀伤细胞(NK 细胞)的数量变化和 CD4~4T 淋巴细胞的活化情况。结果:与 Con A 模型组相比,NCPP 预处理组小鼠血清转氨酶明显降低[ALT 水平从(328±14)U·L~(-1)下降到(104±41)U·L~(-1),AST 水平从(141±9)U·L~(-1)下降到(98±17)U·L~(-1)],肝脏组织 HE 染色显示肝脏病理损伤明显减轻,同时流式细胞术的分析结果显示:与 Con A 模型组相比,小鼠肝脏 NK 细胞数量降低,且 CD4~+T 淋巴细胞的活化受到了明显抑制。结论:NCPP 对 Con A 诱导的小鼠急性肝损伤具有保护作用。其机理可能与抑制 CD4~+T 淋巴细胞活化和拮抗肝脏内 NK 细胞的回流有关。
Objective: To study the protective effect and mechanism of NCPP on Acute Liver Injury induced by Con A in mice. Methods: BALB / c mice were injected intraperitoneally with NCPP (0.01 mg · L -1) and injected with tail vein of Con A (25 μg · g -1) for 7 days to induce acute liver injury in mice. Serum transaminase (ALT / AST) and pathological changes in the liver were measured. At the same time, PBS pretreatment control group and Con A model control group, each group of 8 mice. The number of natural killer cells (NK cells) in the liver and the activation of CD4 ~ 4 T lymphocytes were detected by flow cytometry. Results: Compared with the Con A model group, the levels of serum aminotransferase in the NCPP preconditioning group were significantly decreased (ALT level decreased from (328 ± 14) U · L -1 to (104 ± 41) U · L -1) 1), the AST level decreased from (141 ± 9) U · L -1 to (98 ± 17) U · L -1). The hepatic tissue HE staining showed that the pathological changes of hepatic tissue were relieved. The results of cytology analysis showed that compared with the Con A model group, the number of liver NK cells in mice decreased and the activation of CD4 ~ + T lymphocytes was significantly inhibited. Conclusion: NCPP can protect Con A-induced acute liver injury in mice. The mechanism may be related to inhibiting the activation of CD4 ~ + T lymphocytes and antagonizing the reflux of NK cells in the liver.