目前 ,常规的双孢菇麦粒菌种制作方法是将麦粒经浸泡煮沸后经灭菌用于制种。这一制作流程存在着难以掌握麦粒含水量的缺陷 :当浸泡和煮沸时间过长 (达较高含水量时 )煮沸或高压灭菌过程会使麦粒破裂 ,浆液渗出导致培养基成糊状。接种后菌丝生长缓慢并易结菌块。含水量过小时又 At present, the conventional method for producing Agaricus bisporus is to sterilize the grains for brewing by soaking and boiling. This manufacturing process has the disadvantage that it is difficult to grasp the moisture content of the grain: when the soaking and boiling time is too long (for higher water content), the grain is ruptured by boiling or autoclaving and the oozing out of the slurry causes the medium to become a paste . After inoculation mycelial growth slow and easy to bacteria blocks. When the water content is too small