目的 从杂交瘤细胞的培养上清中纯化低浓度单克隆抗体。方法 采用离子交换层析与凝胶过 滤相结合进行分离纯化。结果 纯化的ＩｇＧ抗体纯度＞９０％，总回收率为３４％。结论 除了杂蛋白的等电点与目 的蛋白相近的情况外，这种方法可应用于低浓度蛋白的分离纯化。 Objective To purify low concentration monoclonal antibodies from the culture supernatant of hybridoma cells. Methods Ion exchange chromatography and gel filtration were used to separate and purify. Results Purified IgG antibody purity> 90%, the total recovery was 34%. Conclusion This method can be applied to the isolation and purification of low concentration protein, except that the isoelectric point of the hybrid protein is similar to the target protein.