用牛血纤溶酶原Separose4B亲和层析方法从蚯蚓粗提物丙酮粉中分离纯化一种纤维蛋白溶解酶。该酶为糖蛋白 ,含糖量约为 1.43 % ,Mr为 30 0 0 0。实验结果表明此酶具有直接溶解纤维蛋白和激活纤溶酶原的间接溶解纤维蛋白的双重作用 ,并对人血凝块有明显的溶解作用。 1%PMSF对酶有显著的抑制作用 ,说明此蚯蚓纤溶酶为典型的丝氨酸蛋白酶类酶 A fibrinolytic enzyme was isolated and purified from the crude acetone extract of alfalfa by affinity chromatography with bovine serum plasminogen Separose 4B. The enzyme is a glycoprotein with a sugar content of 1.43% and Mr of 30000. The experimental results show that this enzyme has the dual function of directly dissolving fibrin and inactivating fibrinolytic activity of plasminogen, and has obvious dissolution effect on human blood clots. 1% PMSF has a significant inhibitory effect on the enzyme, indicating that this chymase is a typical serine protease enzyme