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目的 建立儿茶酚胺和二羟苯乙酸 (Dopac)的毛细管电泳检测方法。 方法 区带毛细管电泳测定儿茶酚胺和 Dopac分离缓冲液为含 5 7mm ol/ L Na H2 PO4,0 .2 m mol/ L EDTA,1.2 mmol/ L HSA和 8%甲醇的缓冲溶液 p H =3.2 ,紫外检测波长为 2 0 0 nm。 结果 儿茶酚胺和 Dopac的分离效果和重现性良好 ,灵敏度可达 5× 10 - 9g/ ml,在 0 .0 1～ 0 .32 μg/ m l浓度范围线性关系好。 结论 本方法用于儿茶酚胺和 Dopac的分析具有快速、简便、耗材少的优点。
Objective To establish a capillary electrophoresis method for the determination of catecholamines and dophenylacetic acid (Dopac). Methods The determination of catecholamines and Dopac in the zone by capillary electrophoresis was carried out in a buffer solution containing 5 7 mm ol / L Na H2 PO4, 0.2 mol / L EDTA, 1.2 mmol / L HSA and 8% methanol p H = 3.2. The detection wavelength is 200 nm. Results The separation and reproducibility of catecholamines and Dopac were good with a sensitivity of 5 × 10 -9 g / ml and a good linear relationship at the concentrations of 0. 01-0. 32 μg / ml. Conclusion The method for the analysis of catecholamines and Dopac has the advantages of fast, simple, less consumables.