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目的:探讨胃癌组织中血管内皮细胞生长因子C(vascular endothelial growth factor-c,VEGF-C)与肿瘤浸润性树突状细胞(tumor infiltrating dendritic cells,TIDC)在胃癌浸润转移中的作用及两者的关系。方法:52例胃癌石蜡标本选自重庆医科大学附属第一医院外科2004年9月至2006年5月手术切除的病例,运用免疫组化法检测胃癌组织中VEGF-C表达及S-100蛋白标记的TIDC的浸润程度,并探讨两者的相关性。结果:(1)在低分化和高、中分化胃癌中,VEGF-C阳性表达率分别为75.86%和44.23%(P<0.05);在VEGF-C阳性、阴性组中,浸润至浆膜的病例分别占72.41%和34.78%(P<0.01),有淋巴结转移的病例分别占93.10%和60.87%(P<0.01),Ⅲ、Ⅳ期胃癌的病例分别占75·86%和47.83%(P<0.05)。(2)在低分化和高、中分化胃癌中,TIDC高度浸润分别占34.48%和52.17%(P>0.05);在TIDC高度浸润、低度浸润组中,浸润至浆膜的病例分别占36.36%和70.00%(P<0.05),有淋巴结转移的病例分别占63.64%和90.00%(P<0.05),Ⅲ、Ⅳ期胃癌病例分别占45.45%和76.67%(P<0.05)。(3)VEGF-C阳性、阴性胃癌中,TIDC高度浸润率分别为27.59%和60.87%(P<0.05),提示VEGF-C可能有抑制TIDC的作用。结论:VEGF-C能够促进胃癌浸润和转移,而高度浸润的TIDC可能有抑制胃癌浸润转移的作用。胃癌组织中VEGF-C影响TIDC的浸润程度,从而可能影响宿主的抗肿瘤免疫能力和肿瘤的浸润转移。
Objective: To investigate the role of vascular endothelial growth factor-c (VEGF-C) and tumor infiltrating dendritic cells (TIDC) in the invasion and metastasis of gastric cancer Relationship. Methods: Fifty-two cases of gastric cancer paraffin specimens were selected from the surgical resections from September 2004 to May 2006 in the First Affiliated Hospital of Chongqing Medical University. The expressions of VEGF-C and S-100 protein in gastric cancer tissues were detected by immunohistochemistry TIDC infiltration, and to explore the correlation between the two. Results: (1) The positive rate of VEGF-C expression was 75.86% and 44.23% respectively (P <0.05) in poorly differentiated and moderately differentiated and moderately differentiated gastric cancer. In VEGF-C positive and negative group, the expression of VEGF- Cases accounted for 72.41% and 34.78% respectively (P <0.01), cases with lymph node metastasis accounted for 93.10% and 60.87% respectively (P <0.01), cases of stage Ⅲ and Ⅳ gastric cancer accounted for 75.86% and 47.83% <0.05). (2) In poorly differentiated and high-grade, moderately-differentiated gastric cancer, TIDC highly infiltrates accounted for 34.48% and 52.17% respectively (P> 0.05). In infiltrating TIDC and low infiltrating groups, infiltration into serosa accounted for 36.36% % And 70.00%, respectively (P <0.05). The cases with lymph node metastasis accounted for 63.64% and 90.00% respectively (P <0.05), and the cases with stage Ⅲ and Ⅳ gastric cancer accounted for 45.45% and 76.67% respectively (P <0.05). (3) The high rate of TIDC infiltration was 27.59% and 60.87% (P <0.05) respectively in VEGF-C positive and negative gastric cancers, suggesting that VEGF-C might inhibit TIDC. Conclusion: VEGF-C can promote the invasion and metastasis of gastric cancer, while highly infiltrated TIDC may inhibit the invasion and metastasis of gastric cancer. VEGF-C in gastric cancer affects the infiltration of TIDC, which may affect the host’s anti-tumor immunity and tumor invasion and metastasis.