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目的 探讨超排卵周期卵泡发育数与颗粒细胞胰岛素样生长因子Ⅰ受体 (IGF ⅠR)表达的关系。方法 根据取卵时卵泡发育数不同 ,将卵巢对超排卵药物的反应分为低反应型 (卵泡数≤3个 )、中反应型 (卵泡数 4～ 13个 )和高反应型 (卵泡数≥ 14个 ) ,分别为 11、16、12例。采用逆转录聚合酶链反应 (RT PCR)和蛋白印迹 (Western blot)法分别测定 3种反应类型的卵泡颗粒细胞IGF ⅠRmRNA的表达量和IGF ⅠR的含量。结果 低反应型IGF ⅠRmRNA表达量显著低于中、高反应型[相对积分值分别为 1 70± 0 2 3( x±s,下同 )、2 92± 0 49和 4 2 2± 1 5 0 ,P <0 0 5 )。低反应型与中反应型及高反应型相比 ,IGF ⅠR含量也减少 (相对吸光度分别为 1 32± 0 31、2 0 1± 0 72和 4 39±2 30 ) ,差异有显著性 (P <0 0 5 )。结论 颗粒细胞IGF ⅠR含量可能影响超排卵周期卵泡发育数
Objective To investigate the relationship between the follicle development and the expression of insulin-like growth factor receptor (IGF-ⅠR) in granulosa cells. Methods The ovarian response to superovulation was divided into three groups according to the number of ovarian follicles: the number of follicles (≤ 3 folds), the number of medium reactive follicles (follicles 4 ~ 13) 14), respectively, 11,16,12 cases. RT-PCR and Western blot were used to determine the expression of IGF-IR mRNA and IGF-IR of three types of granulosa cells. Results The expression of low-response IGFR mRNA was significantly lower than that of moderate-high responders (relative integral values were 1 70 ± 0 2 3 (x ± s, the same below), 2 92 ± 0 49 and 4 2 2 ± 150 , P <0 0 5). Compared with the medium and high responders, the low-response IGF-IR decreased (relative absorbances were 1 32 ± 0 31, 2 0 1 ± 0 72 and 4 39 ± 2 30, respectively) (P <0 0 5). Conclusion The granulocyte IGF Ⅰ R content may affect the number of follicle development during superovulation