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目的:观察银杏叶提取物(GBE)对急性心肌梗死(AMI)后转化生长因子-β1表达的影响。方法:将30只雄性SD大鼠随机分为假手术组,模型组,银杏叶组3组。按文献方法假手术组左冠状动脉前降支下只穿线不打结,另外两组均结扎左冠状动脉前降支。其中银杏叶组腹腔注银杏叶提取物2 m L/kg,假手术组和模型组分别腹腔注射等量生理盐水,连续注射4周后,HE染色观察心肌组织形态,免疫组织化学检测转化生长因子-β1(TGF-β1)、I型胶原蛋白的表达。结果:银杏叶治疗组心功能明显优于模型组,心脏重量/体重、左室重量/体重低于模型组(P<0.05),TGF-β1、I型胶原蛋白的表达明显低于模型组(P<0.01)。结论:银杏叶提取物能够通过降低TGF-β1、I型胶原蛋白的表达抑制急性心肌梗死引起的心室重构。
Objective: To observe the effect of GBE on the expression of transforming growth factor-β1 after acute myocardial infarction (AMI). Methods: Thirty male SD rats were randomly divided into sham operation group, model group and Ginkgo biloba group. According to the literature method, the left anterior descending coronary artery of the sham-operation group was threaded only without knots, and the other two groups were ligated with the left anterior descending coronary artery. The Ginkgo biloba group was given intraperitoneal injection of Ginkgo biloba extract (2 m L / kg), and the rats in sham operation group and model group were injected intraperitoneally with normal saline. After 4 weeks of continuous injection, the morphology of myocardial tissue was observed by HE staining and the expression of transforming growth factor -β1 (TGF-β1), type I collagen. Results: The cardiac function of Ginkgo biloba group was significantly better than that of model group. The heart weight / body weight and left ventricular weight / body weight were lower than those in model group (P <0.05). The expression of TGF-β1 and collagen I in model group was significantly lower than that in model group P <0.01). Conclusion: Ginkgo biloba extract can inhibit ventricular remodeling induced by acute myocardial infarction by decreasing the expression of TGF-β1 and type I collagen.