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目的对杭州市首例本地感染登革热病例开展调查及病原分子学溯源。方法对首例本地感染登革热病例开展流行病学调查,采集患者血清进行登革病毒核酸和抗体检测。提取病毒核酸后扩增E基因并测序,利用生物信息学软件进行多序列比对排列及构建进化树。结果该病例的登革病毒核酸及Ig M抗体均为阳性,基因序列比对及进化分析,病毒株为登革病毒1型GⅣ亚型,与韩国2007年1例从菲律宾旅游输入病例分离的病毒株亲缘关系最近,核苷酸和氨基酸同源性为98.9%和99.6%,与菲律宾2010年的2株病毒核苷酸和氨基酸同源性为98.6%和99.6%,病毒株来源于菲律宾的可能性较大。结论杭州市首例本地感染登革热病例可能是由菲律宾旅游归国的输入性病例引发的二代病例。
Objective To investigate and trace the origin of dengue fever in Hangzhou. Methods Epidemiological investigation of the first local case of dengue fever was conducted. Serum was collected for dengue virus nucleic acid and antibody detection. E gene was extracted after amplification of viral nucleic acid and sequenced. Bioinformatics software was used to align and construct phylogenetic tree. Results The dengue virus nucleic acid and Ig M antibody were positive, the gene sequence alignment and phylogenetic analysis were performed. The strain was dengue virus type 1 GIV subtype and was isolated from a virus that was imported from a Philippine tourist in 2007 in Korea The phylogenetic relationships between the two strains were 98.9% and 99.6% for nucleotide and amino acid homology, 98.6% and 99.6% for nucleotide and amino acid sequences of the two strains of the Philippines in 2010, and the possible origin of the strain in the Philippines Sex larger. Conclusions The first local case of dengue fever in Hangzhou may be the second generation caused by imported cases of Philippine tourist return.