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目的 研究ＨＣＶ对人Ｂ淋巴细胞的感染，建立ＨＣＶ感染的丙型肝炎患者Ｂ淋巴细胞模型（ＣＢＣＬ），并进行ＨＣＶ的形态学研究。方法 用ＥＢ病毒转化Ｂ细胞建立Ｂ细胞系，以逆转录多聚酶链反应（ＲＴ－ＰＣＲ）、免疫组织化学、原位杂交方法检测Ｂ细胞系上清液及细胞内的ＨＣＶ抗原及ＨＣＶ ＲＮＡ，并通过电镜对ＨＣＶ进行形态学研究。结果 细胞系上清液中ＨＣＶ ＲＮＡ呈阳性，细胞内ＨＣＶ抗原及ＨＣＶ ＲＮＡ均呈阳性，电镜观察结果发现细胞内存在６５ｎｍ和１１０ｎｍ圆球型病毒颗粒，并可见病毒芽生形成现象。结论 ＨＣＶ可感染人Ｂ淋巴细胞并在其中复制。病毒在感染细胞胞质空泡部位合成和组装，以芽生方式进入胞质空泡内形成病毒颗粒。
Objective To study the HCV infection of human B lymphocytes and establish a C-infected lymphocyte model of hepatitis C (CBCL) and to study the morphological characteristics of HCV. Methods The B cell line was established by transforming Epstein-Barr virus (B) cell line with Epstein-Barr virus (EBV) and the HCV RNA and HCV RNA in the B cell line supernatant and intracellular were detected by reverse transcriptase polymerase chain reaction (RT-PCR), immunohistochemistry and in situ hybridization Morphological study of HCV by electron microscopy. Results The HCV RNA in the supernatant of the cell line was positive, and the intracellular HCV antigen and HCV RNA were positive. The results of electron microscopy showed that there were nucleocapsid particles of 65nm and 110nm in the cell and the formation of virus budding was observed. Conclusion HCV can infect and replicate human B lymphocytes. The virus is synthesized and assembled in the cytoplasmic vacuolar parts of the infected cells and enters the cytoplasmic vacuoles to form viral particles in a budding manner.