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目的 :定位自发性SLE模型 (NZB×NZW)F1小鼠CD8+ T细胞数量异常的遗传易感基因。方法 :建立 (NZB×NZW )F1×NZW回交小鼠模型 ,采用覆盖小鼠 1 9条常染色体的多态性微卫星遗传标记及数量性状位点分析进行基因定位。结果 :CD8+ T细胞数量异常的易感基因与小鼠第 1条染色体尾端 92 3cM处的微卫星遗传标记D1Mit36肯定连锁 (Lods值 >3) ,且回交小鼠该遗传标记B W型组的外周血淋巴细胞中CD8+ T细胞百分比明显低于W W型组 (P <0 0 0 1 )。结论 :(NZB×NZW )F1小鼠外周血CD8+ T细胞数量异常的候选易感基因位于第 1条染色体尾端 92 3cM附近 ,来源于NZB小鼠。
Objective: To identify the genetic predisposition genes of CD8 + T cells in spontaneous SLE model (NZB × NZW) F1 mice. Methods: A (NZB × NZW) F1 × NZW backcross mouse model was established. The genetic loci were analyzed by using microsatellite markers and quantitative trait loci of 19 autosomal chromosomes in mice. Results: The susceptible gene with abnormal CD8 + T cell number was positively linked with the microsatellite marker D1Mit36 at 92 3cM at the tail of mouse chromosome 1 (Lods value> 3), and the genetic marker BW group The percentage of CD8 + T cells in peripheral blood lymphocytes was significantly lower than that in the WW group (P <0.01). CONCLUSION: The candidate susceptibility gene of CD8 + T cells in peripheral blood of (NZB × NZW) F1 mice is located near 92 3cM of the tail of chromosome 1 and is derived from NZB mice.