论文部分内容阅读
作者使用的Pariorix疫苗株为UrabeAm9株,平均滴度为10~4.7TCID_(50)17名无腮腺炎病史并经ELISA筛选腮腺炎抗体阴性的成人(20~30岁)为免疫对象。每人皮下注射疫苗0.5ml。用ELISA、HI及HIG三种试验测定免疫前及免疫后21、48及62天的血清抗体。ELISA结果以吸收值(血清稀释度1:160)、终点滴度及ELISA单位三种方法表示,试验结果表明三者密切相关,测得的血清阳转率无差别。血清抗体以ELISA滴度≥1:120、HI滴度≥1:10和HIG溶血区直径≥5mm者判为阳性。
The Pariorix vaccine strain used was the Urabe Am9 strain, with an average titer of 10 to 4.7 TCID_ (50) 17 mumps-free adults (20-30 years) immunized against mumps antibody by ELISA screening. Each subcutaneous injection of vaccine 0.5ml. Serum antibodies were measured before and at 21, 48 and 62 days after immunization with ELISA, HI and HIG. ELISA results to absorb the value (serum dilution 1: 160), the end point titer and ELISA units three methods that the test results show that the three are closely related to the measured serum positive rate no difference. Serum antibodies with ELISA titer ≥ 1: 120, HI titer ≥ 1: 10 and HIG hemolysis area diameter ≥ 5mm were sentenced as positive.