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恶性疟原虫裂殖子表面主要蛋白－１，又称Ｐ１９５，与人红细胞膜具有结合作用，这种结合是裂殖子识别红细胞的基础。为了确定Ｐ１９５蛋白与人红细胞的结合位点，我们在大肠杆菌中分８段表达了Ｐ１９５蛋白。各段表达蛋白经过提纯与复性后，用胶体金标记。标记后的各段蛋白分别与人红细胞共孵育。然后将此红细胞加入到银显影液中。结果发现，红细胞在与一段Ｐ１９５蛋白（Ｍ６，氨基酸序列为３８４－５９５）共孵育并加入到银显影液中后，红细胞表面出现黑色银沉淀。而红细胞在与其它各段Ｐ１９５蛋白进行同样操作后，红细胞表面没有出现黑色银沉淀。这表明经胶体金标记后的Ｍ６与人红细胞具有结合作用。Ｍ６所对应的区域可能就是Ｐ１９５蛋白的红细胞结合区域。
Plasmodium falciparum merozoite surface protein-1, also known as P195, has a binding role with human erythrocyte membrane, which is the basis of merozoite recognition of red blood cells. To determine the binding site of P195 protein to human erythrocytes, we expressed P195 protein in E. coli in eight segments. The expressed protein in each stage was purified and refolded, and then labeled with colloidal gold. The labeled proteins were incubated with human erythrocytes separately. This red blood cell is then added to the silver developer solution. The results showed that when erythrocytes co-incubated with a piece of P195 protein (M6, amino acid sequence of 384-595) and added to silver developer solution, black silver precipitates on the surface of erythrocytes. The red blood cells in the other sections of P195 protein after the same operation, no black silver precipitation on the surface of erythrocytes. This indicates that M6, which is labeled with colloidal gold, binds to human erythrocytes. The region corresponding to M6 may be the erythrocyte binding region of P195 protein.