[Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patula L. [Method] By using tissue culture technology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patula L. [Result] Shoot tips or stem segments of T. patula L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing 0.1 mg/L NAA and 1.5 mg/L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was the maximum (97%) in 1/2MS medium containing 0.2 mg/L NAA, and the root system was relatively developed. [Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L. [Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patula L. [Method] By using tissue culture technology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patula L. [Result] Shoot tips or stem segments of T. patula L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing 0.1 mg / L NAA and 1.5 mg / L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was The maximum (97%) in 1 / 2MS medium containing 0.2 mg / L NAA, and the root system was relatively developed. [Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L.