目的 :检测胚胎甲状腺中是否表达褪黑素受体。 方法 :取人胚胎甲状腺 ,抽提总 RNA并用 RT- PCR方法检测其 m R-NA,阳性产物用自动测序仪测序。 结果 :人胚胎甲状腺总 RNA以 MT1及 MT2两种引物进行的 RT- PCR产物电泳呈现阳性条带 ,测序结果显示扩增产物与人类褪黑素受体 c DNA相吻合。结论 :人胚胎甲状腺组织中存在有褪黑素 MT1,MT2两种受体的 m RNA,表明该组织中有褪黑素 MT1,MT2受体的表达 Objective: To detect whether melatonin receptor is expressed in embryonic thyroid. Methods: The human embryonic thyroid gland was sampled and the total RNA was extracted. The m R-NA was detected by RT-PCR. The positive products were sequenced by automatic sequencing. Results: RT-PCR products of human embryonic thyroid total RNA showed positive bands with MT1 and MT2 primers, and the sequencing results showed that the amplification products were consistent with human melatonin receptor c DNA. Conclusion: The m RNA of melatonin MT1 and MT2 receptor exist in human embryonic thyroid tissue, indicating that the expression of melatonin MT1 and MT2 receptor