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目的观察MUC1的模拟表位肽对表达人MUC1 T739荷瘤小鼠的治疗作用。方法建立稳定表达人MUC1 T739荷瘤小鼠,培养T739小鼠成熟树突状细胞,荷瘤小鼠随机分为4组,用负载模拟表位肽成熟DC免疫表达人MUC1 T739荷瘤小鼠,第1组皮下注射等渗盐水,第2组注射空DC,第3组注射负载1号肽的DC,第4组注射负载2号肽的DC,测量肿瘤大小,计算肿瘤体积,称瘤重,并观察小鼠存活期。结果两组DC+肽免疫的小鼠瘤体积减小明显,与1、2组相比差异非常显著(P<0.01)。平均瘤重1、2组与3、4组相比差异非常显著(P<0.01)。第1～4组荷瘤鼠的生存期分别为(34.6±3.507)d,(35.2±3.564)d,(58.4±5.595)d,(59±6.819)d,3、4两组荷瘤小鼠生存期较1、2组明显延长,差异有显著性意义(P<0.01)。结论 MUC1模拟表位肽能明显抑制表达人MUC1 T739荷瘤小鼠肿瘤生长,显著延长T739荷瘤小鼠生存时间。
Objective To observe the therapeutic effect of MUC1 mimotope peptide on human MUC1 T739 tumor-bearing mice. Methods MUC1 T739 tumor-bearing mice were stably expressed, and mature T739 mice were cultured. Tumor-bearing mice were randomly divided into 4 groups. Human MUC1 T739 tumor-bearing mice were immunostimulated with mature DCs carrying mimotope peptide. Group 1 was injected subcutaneously with isotonic saline, Group 2 was injected with DC, Group 3 was injected with DC loaded with peptide # 1, Group 4 was injected with DC loaded with peptide # 2, tumor size was measured, tumor volume was calculated, Mice were observed for survival. Results The tumor volume of mice immunized with DC + peptide decreased significantly compared with that of mice in group 1 and 2 (P <0.01). The average tumor weight 1, 2 groups and 3,4 groups compared to the difference was significant (P <0.01). The survival time of mice in groups 1 to 4 were (34.6 ± 3.507) d, (35.2 ± 3.564) d, (58.4 ± 5.595) d, (59 ± 6.819) d, The survival time was significantly longer than that in group 1 and 2, with significant difference (P <0.01). Conclusion MUC1 mimotope peptide can significantly inhibit tumor growth in MUC1 T739-bearing mice and significantly prolong the survival of T739-bearing mice.